Abstract. Flores, J. R.; S. A. Catalano & G. M. Suárez. 2017. Cladistic analysis of the family Cryphaeaceae (Bryophyta) with emphasis on Cryphaea: a study based on a comprehensive morphological dataset. Darwiniana, nueva serie 5(1): 51-64.
The first comprehensive phylogenetic analysis of the Cryphaeaceae (Bryophyta), a pleurocarpic moss family, is conducted on the basis of morphological characters. The data set comprised 73 characters: 10 continuous and 63 discrete. Taxon sampling involved nine genera and 46 species of Cryphaeaceae, 32 species belonging to Cryphaea. Outgroup sampling included 23 species from 21 genera and 13 families of pleurocarpous mosses. The phylogenetic analyses were conducted using parsimony as the optimality criterion following an implied weighting approach. The results did not support the monophyly of Cryphaeaceae as it excluded Dendroalsia abietina from the family. The clade composed of the remaining genera (clade A) was diagnosed by a short seta (0.26-0.30 mm), costa present throughout the innermost perichaetial bract, conical operculum and appressed leaves in dry condition. The analyses furthermore recovered Cryphaea as paraphyletic and Dendrocryphaea as polyphyletic. Cryphaea included Schoenobryum concavifolium, Cyptodontopsis leveillei, and Dendrocryphaea lamyana which were thereby separated from the other species of Dendrocryphaea. Character mapping revealed that, as a consequence of the unexpected placement of crucial species, diagnosis should be considerably modified.
Keywords. Implied weighting; parsimony; pleurocarpous mosses; systematics; taxonomy.
Resumen. Flores, J. R.; S. A. Catalano & G. M. Suárez. 2017. Análisis cladístico de la familia Cryphaeaceae (Bryophyta) con énfasis en Cryphaea: un estudio basado en un conjunto integral de datos morfológicos. Darwiniana, nueva serie 5(1): 51-64.
El primer análisis filogenético de Cryphaeaceae (Bryophyta), una familia de musgos pleurocárpicos, se lleva a cabo sobre la base de caracteres morfológicos. El conjunto de datos consta de 73 caracteres: 10 continuos y 63 discretos. El muestreo de taxa incluye 9 géneros y 46 especies de Cryphaeaceae, 32 especies pertenecientes a Cryphaea. El grupo externo, incluye 23 especies de 21 géneros y 13 familias de musgos pleurocarpous. Los análisis filogenéticos se realizaron utilizando parsimonia bajo pesos implicados. Los resultados no apoyan la monofilia de Cryphaeaceae ya que excluye a Dendroalsia abietina de la familia. El clado compuesto por los géneros restantes (clado A) fue diagnosticado por una seta corta (0,26-0,30 mm), costa presente en todas las hojas periqueciales internas, opérculo cónico y hojas adpresas al estado seco. Los análisis además recuperaron Cryphaea como parafilético y Dendrocryphaea como polifilético. Cryphaea incluye Schoenobryum concavifolium, Cyptodontopsis leveillei, y Dendrocryphaea lamyana que se separaron de las otras especies de Dendrocryphaea. El mapeo de caracteres reveló que, como consecuencia de la ubicación inesperada de especies cruciales, la diagnosis debería ser considerablemente modificada.
Palabras clave. Parsimonia; pesos implicados; musgos pleurocárpicos; sistemática; taxonomía.
INTRODUCCIÓN
In recent years, traditional systematic schemes in bryology were subjected to a harsh review. Although some pre-cladistics schemes were reconsidered by analysing morphological data sets (Hedenäs, 1994, 1996 a,b), major taxonomic modifications are currently established based on the results of molecular studies (Capesius & Stech, 1997; Cox & Hedderson, 1999). Pleurocarpous mosses, a distinctive group of bryophytes, were reviewed on the basis of both morphology (Buck, 1988; Hedenäs, 1994, 1995, 1996 a, b) and molecules (Buck et al., 2000; De Luna et al., 1999). However, the monophyly of some internal groups has been scarcely evaluated. This is the case of the family Cryphaeaceae Schimp. Although this taxon is considered to be crucial for the understanding of the evolution of pleurocarpy (Buck et al., 2000; LaFarge-England, 1996), no comprehensive phylogenetic study has been conducted in order to test its monophyly. Proposals about its monophyly and phylogenetic placement were always done in the context of higher taxonomic level phylogenies, with a limited taxon sampling for this family (Buck et al., 2000; Cox et al., 2010; Maeda et al., 2000; Quandt et al., 2004).
Historically, the taxonomy of Cryphaeaceae as well as its most diverse genus, Cryphaea D. Mohr, has been challenging. Such a conundrum is reflected in the copious number of studies which propose conflicting schemes of classification at both family and genus level (e.g. Brotherus, 1903; Fleischer, 1906, 1914; Flores & Suárez, 2014; Robinson, 1972; Schimper, 1856; Suárez & Schiavone, 2004, 2010; Table 1). As pointed out by Buck (1998) and Rao (2000, 2001), taxa within Cryphaeaceae are highly variable and divergent in aspect. Consequently, the definition of these taxa is not an easy task. Plants within the family have been characterized as species which constitute tufts or have pendulous strands; stems are differentiated in a creeping leafless primary stem and an erect or pendent secondary stem: leaves vary in size and form, from ovate leaves with acute apex and plane margins to acuminate with serrulate margins. Manuel (1974, 1981, 1982) proposed a combination of three characters to distinguish the main genera within the Cryphaeaceae. Nevertheless, some of these characters were not helpful to separate problematic genera (Manuel, 1981, 1982), such as Cyptodon (Broth.) Paris & Schimp. ex M. Fleisch.and Dendrocryphaea Paris & Schimp. ex Broth. A further taxon with dubious taxonomic position identified by Manuel was Cyptodontopsis Dixon, which was considered as a “transitional” genus between Dencrocryphaea and Cyptodon. Despite some features being constant throughout the family (Maeda et al., 2000).
Cryphaeaceae were traditionally regarded as members of the Leucodontales based on their pleurocarpic habit and a sympodial branching pattern (Buck, 1998). However, Buck et al. (2000) found evidence to include Leucodontales as part of Hypnales, so Cryphaeaceae are classified within the latter order in the system proposed by Goffinet et al. (2009). Until the last decade, relatively few advances were done to clarify the phylogenetic status of the family. Maeda et al. (2010) carried out the most extensive molecular phylogenetic analysis of Leucodontineae up to that moment. That study included 25 taxa of Leucodontineae and three genera of Cryphaeaceae, and obtained a close relationship between the species of Cryphaeaceae and the family Leucodontaceae. By analysing three molecular markers (ITS2, trnL-F and psbT-H) Quandt et al. (2004) transferred Cryphaeophilum molle (Dusén) M. Fleisch. from Meteoriaceae to Cryphaeaceae. Recently, Cox et al. (2010) carried out one of the largest studies concerning Bryophyta in terms of taxonomic sampling, where a considerable number of pleurocarpous families were resolved as paraphyletic.
Cryphaea, widely distributed throughout the Old and New World (Rao, 2001), is the most specious genera of the family Cryphaeaceae. Weber (1813) gave account of the mitriform calyptra of Cryphaea as a defining feature. Later, Bridel (1819) took into account the immersed capsule, double peristome of 16 teeth and the smooth calyptra. After several taxonomic changes, Brotherus (1905, 1924) recognised ovate to elliptic laminar cells, erect secondary stem, capsule shape and spore size as diagnostic generic characters. Following Gradstein et al. (2001), the genus Cryphaea can be identified by its erect habit, lateral sporophytes, ovoid almost sessile capsules, and papillose exostome teeth. In addition, Buck (1998) had previously suggested unicostate leaves as a distinctive feature of the genus. However, some of these characters are highly variable. In this sense, pseudoparaphyllia were described as foliose (Buck, 1998) or as filamentous (Rao, 2001). Therefore, an extensive morphological study should be conducted in order to consider the wide variability of the genus, and to evaluate the phylogenetic information content of that variation.
Until Rao’s (2001) monographic studies, no phylogenetic analysis had been conducted in Cryphaea. Based on that cladistic analysis and previous taxonomical studies, Rao adjusted the definition of Cryphaea by including only species with diplolepidous peristome and epiphytic habit. In that study, Rao also retrieved the monophyly of Cryphaea and relocated Cryphaea lamyana (Mont.) Müll. Hal. to Dendrocryphaea lamyana (Mont.) P. Rao, the latter decision being grounded on the aquatic habit and the cladocarpous position of the sporophyte of C. lamyana. Although the meticulously labour of Rao (Rao 2000, 2001; Rao & Enroth, 1999) is, undoubtedly, a key for the understanding of Cryphaea taxonomy, some cautions must be taken about the results of the phylogenetic hypothesis (Rao, 2001) as the analysis presented a limited taxon sampling and included a very restricted number of outgroups.
Because of the possible relevance of Cryphaeaceae for the study of pleurocarpy and the lack of a comprehensive morphological phylogenetic analysis, the purpose of the present paper is to evaluate for the first time the monophyletic status of Cryphaeaceae focusing on Cryphaea. The results presented herein provide a first insight into the status of and relationships within the family Cryphaeaceae based on an extensive morphological dataset.
MATERIALS AND METHODS
Taxon and Character Sampling. The present study included 69 species (46 ingroup species of Cryphaeaceae and 23 outgroup taxa; Table 2). The outgroup taxa include 12 families and 17 species from the Hypnales and, two families and six species from Hookeriales. Among these, Cyclodictyon lorentzii (Müll.Hal.) W. R. Buck & Schiavone was selected for rooting. Ingroup taxa include all the genera of Cryphaeaceae (Goffinet et al., 2009), and a total of 46 species, 32 of them belonging to Cryphaea. Of these, eight species are included in a morphological phylogenetic analysis for the first time: Cryphaea lorentziana Müll. Hal., Cryphaea furcinervis Müll. Hal., Cryphidium leucocoleum (Mitt.) A. Jaeger., Dendrocryphaea tasmanica (Mitt.) Broth., Dendrocryphaea cuspidata (Sull.) Broth., Dendrocryphaea latifolia D. G. Griffin, Gradst. & J. Aguirre, Dendrocryphaea gorveana (Mont.) Paris & Schimp. and Cryphaeophilum molle. For voucher information see Supplementary appendix.
Character sampling involved 73 morphological characters (44 gametophytic, 29 sporophytic). Eighteen multistate characters were considered as ordered, based on the different degrees of similarity observed among their states (Lipscomb, 1992). Ten characters, which represented measurements of different structures, were analysed as continuous characters (Goloboff et al., 2006). This is a source of information not usually included in phylogenetics studies of mosses. Continuous characters were standardized in such a way that the full range of each character was equal to one step in a discrete character. Finally, a single character (character 21; perichaetial position) was scored as a Sankoff character (Sankoff & Rousseau, 1975), with symmetrical cost changes among states (1 step from cladocarpous to pleurocarpous and, 2 steps from acrocarpous to cladocarpous/pleurocarpous). Most traits were directly observed and scored from specimens. In those cases where specimens (or parts) were not available, the matrix was completed with data from specialised bibliography (Buck, 1998; Rao, 2000, 2001; Rao & Enroth, 1999). Two species were completely scored from literature: Daltonia stenophylla Mitt. and Leskeodon palmarum (Mitt.) Broth. (Buck, 1998). The final dataset and complete descriptions of the new characters are in Supplementary appendix (Tables S1-S2). Further information on results, character scoring and specimen images is freely available at Morphobank webpage (http://morphobank.org/permalink/?P2411)
Cladistic Analyses. Phylogenetic analyses were run in TNT 1.1 (Goloboff et al., 2008a; Version 1.1 (February 2014), a free access software, considering parsimony as optimality criterion. Tree searches were performed under implied weighting (Goloboff, 1993). This approach, which weights characters during searches according to their homoplasy, has shown to enhance the results of morphological data analyses when compared with equal-weighted parsimony (see discussion in Goloboff et al., 2008b). Given the lack of an objective criterion to choose a specific strength for downweighting homoplasy (concavity value, K), the analyses were repeated considering ten different concavities (K: 4.344 – 9.231; K selection followed Mirande, 2009; Fig. 1). The main taxonomic results were derived from the topology which remained the less sensitive to parameter variation (concavity value). That is, that topology which was recovered with more frequency across the concavity range was regarded as the final tree. However, in order to achieve more conservative conclusions, the strict consensus from the complete set of trees was also employed. In all cases, the search strategy involved 8 replicates of: 3 rounds of fusing, 10 cycles of drifting and ratchet, and sectorial searches (Goloboff, 1999), hitting at least 3 times the best score (command “xmult”). These cladograms were again submitted to TBR, keeping up to 1.000 trees in memory. Node support was measured with standard Bootstrapping (Felsenstein, 1985), and Jackknifing (Farris et al., 1996).
Constrained searches. Constrained searches (forcing some groups to be monophyletic) were performed in order to evaluate the optimality of taxonomic groups previously proposed in the literature but that were not found as monophyletic in the present analyses. The difference in scores between the best tree with and without the group under consideration is a measure of how much evidence is contradicting that grouping in the best tree. Since making direct comparisons in terms of fit is not easy to be interpreted, differences in fit were translated to the number of mean homoplastic character with an extra step added (that is, x̄ + 1). A mean homoplastic character (x̄) is obtained as the tree length divided by the number of characters. By taking the ratio between the fit differences of topologies (F unconstrained, F constrained) and mean homoplasious characters (Fx̄, Fx̄+1) suboptimality is conceived in light of how many mean homoplastic characters which gained one step are needed to explain that suboptimality [(F unconstrained - F constrained)/(F x̄ - F x̄ + 1)].
All these procedures (concavity exploration, topology stability assessment and constrained searches) were implemented into TNT scripts available upon request. A detailed description of this methodology is found in the Supplementary appendix.
Character mapping. In order to explore the behaviour of the diagnosing characters, these were mapped along the stable topology. The optimization of continuous characters considered the complete range of reconstructions. That is, in cases where the character had multiple reconstructions at internal nodes, the complete set of possible reconstructions (implied by the extreme values of the optimization) was scrutinised. Thus, overall positive or negative changes at internal nodes were only considered as such when the complete set of reconstruction indicated an increment or decrement, respectively.
RESULTS
Character analysis
Except for the previously excluded species, observation of the available specimens verified Rao’s (2001) scoring. Although Rao (2001) did not include Cryphaea furcinervis in his analysis, he appealed to the species’ protologue for considering its capsule as an oval capsule (character 32). The shape of the capsule of C. furcinervis, examined in specimens from Argentina, did not agree with such a description. Scoring was made in accordance with available from type and fresh material of C. furcinervis. In addition, other characters were modified in order to include new observations of outgroup taxa not previously incorporated in the matrix. In some cases character state definitions were modified, while in other characters new states were added. This is the case of character 14 and 15.
Phylogenetic analysis
Relationships within Cryphaeaceae
The exploration of concavity values concluded in one fully resolved tree per K value, and their distortion ranged from 42.96 to 53.58 (Table 3). A single topology, obtained in six out of ten K values, was found to be the most frequent across the entire range of concavities (i.e. the least sensitive; Fig. 2). Such a topology retrieved a non-monophyletic Cryphaeaceae, and a close association between Dendroalsia abietina (Hook.) E. Britton ex Broth. and Orthostichopsis tenuis (A. Jaeger) Broth. (Fig. 2). Cryphaeophilum molle appeared as the sister taxon of the remaining genera of the family with the exception of D. abietina (clade A - Fig. 2). Within clade A, a basal dichotomy separated four of the five Dendrocryphaea species and Cryphidium leucocoleum (clade B, Fig. 2) from the remaining species (clade C, Fig. 2). The recently recognised D. lamyana (Rao, 2001) was nested within clade C, more closely related to Cryphaea ovalifolia (Müll. Hal.) A. Jaeger than to any other species of Dendrocryphaea. This rendered the genus Dendrocryphaea polyphyletic as is currently circumscribed. Within clade C, the clades Dendropogonella-Pilotrichopsis and Sphaerotheciella successively branched as sister groups to Cryphaea, Cyptodontopsis, Dendrocryphaea lamyana and Schoenobryum (Fig. 2). The main genus, Cryphaea, was not monophyletic as it included the remaining genera of Cryphaeaceae.
Relationship between Cryphaeaceae and outgroup families
The status and relationship among pleurocarpous families have been hardly elucidated (Buck et al., 2000; Cox et al., 2010). Consequently, no family could be convincingly proposed as the sister group of the Cryphaeaceae (Maeda et al., 2000; Cox et al., 2010). In the present study, many of these pleurocarpous families were non-monophyletic. Indeed, half of the families represented by more than a single species resulted non-monophyletic. In accordance with this pattern, a paraphyletic Pterobryaceae constituted the sister taxon of Cryphaeaceae (Fig. 2). Even though the support values were low, most of the clades found within the Cryphaeaceae-Pterobryaceae node were highly stable and were recovered under more than six concavity values (Fig. 3).
Constrained searches
In spite of the general low support values, forcing monophyly of para- or polyphyletic taxa had a slight to notably impact on the tree score. Forcing Cryphaeaceae monophyly implied that 2.28 characters added an extra step. Similarly, monophyly of Dendrocryphaea required an extra step in 2.31 characters. However, in the case of Cryphaea, a considerable number of characters added a further step (6.63). Likewise, resultant trees were also suboptimal when the monophyly of the outgroup families was forced. Monophyly of Thuidiaceae implied the smallest difference on the global tree score (1.34). The monophyletic status of Pterobryaceae affected the overall score slightly more (2.20). Surprisingly, the monophyly of Daltoniaceae turned out to be strongly suboptimal (4.91). This difference in optimality was certainly unexpected given that Daltonia stenophylla was the species with the maximum number of missing entries, which do not add extra steps when they are optimised.
Character mapping
Character mapping indicated that four characters diagnosed clade A (Cryphaeaceae, excluding D. abietina; Fig. 4): seta length, costa of the internal perichaetial bracts present, operculum shape, and leaf position when dry. When mapped, the length of the seta showed a progressive decrease. However, at the base of clade A, the seta length suffered a considerable shortening. Consequently, a seta shorter than 0.30 mm diagnosed clade A. A costa present throughout the internal perichaetial bract also characterised this node. However, at internal nodes, the character state reverts as a costa disappearing downwards the bract or completely absent from it. A conical shape of the operculum furthermore diagnosed the clade A, and only Neckeraceae and Herpetineuron toccoae (Sull. & Lesq.) Cardot depicted the same state outside clade A. A rostrate condition was secondarily present in some species of Cryphaea. Finally, a markedly appressed leaf position was a consistent feature of all the Cryphaeaceae. Nevertheless, this state also arose among the outgroups in Meteorium deppei (Hornsch. ex Müll. Hal.) Mitt., Thuidium delicatulum (Hedw.) Schimp., Forsstroemia coronata (Mont.) Paris, Leucodon julaceus (Hedw.) Sull. and Thelia hirtella (Hedw.) Sull.
DISCUSSION
Phylogenetic patterns and Taxonomy
In the present study, phylogenetic relationships of the pleurocarpic family Cryphaeaceae were analysed, with a special focus on Cryphaea. Because exhaustive or explicit testing of the diagnosis features of this group are infrequent, most of the results presented here impact not only the taxonomical status of genera and families but also their morphological concepts.
One of the most important results obtained in this study was the corroborated non-monophyly of Cryphaeaceae. Until the molecular work of Cox et al. (2010), there was scarce evidence about the status of this family (Buck et al., 2000; Maeda et al., 2000; Quandt et al., 2004). In the study carried out by Maeda et al. (2000), Cryphaeaceae were represented by only three taxa, Cryphaea sinensis E.B. Bartram, Cyptodontopsis obtusifolia (Nog.) Nog., and Pilotrichopsis dentate (Mitt.) Besch.; and its conclusion clarified much of the systematics problems of the Leucodontineae suborder. However,that analysis was unable to elucidate the relationship among the genera included in the Cryphaeaceae. Cox et al. (2010), who provided the most solid evidence about the nature of Cryphaeaceae, found a polyphyletic Cryphaeaceae due to the exclusion of Dendroalsia and Pilotrichopsis. Besides the inclusion of Pilotrichopsis within the clade A, the results presented here were quite in agreement with those of Cox et al. (2010). Concordantly, a monophyletic circumscription of Cryphaeaceae would require excluding, at least, the genus Dendroalsia. Pilotrichopsis, on the other hand, was well nested within the clade A. Although this fact is not contradicted by the results of Maeda et al. (2000), it is significantly opposed to that of Cox et al. (2010) where Pilotrichopsis was placed within Pterobryaceae. Based on the results of Maeda et al. (2000), Cox et al. (2010) and the present ones, it is likely that a more exhaustive sampling of genera within the Pterobryaceae and Leucodontaceae will help to elucidate the position of Pilotrichopsis.
Establishing the relationship of the Cryphaeaceae with other families has also been a difficult task (Maeda et al., 2000). As mentioned by Maeda et al. (2000), several authors have proposed alternative hypotheses. Brotherus (1924) and Manuel (1982) indicated that Leucodontaceae and Cryphaeaceae could be related because of their laminar cell shape and the upright position of the capsule. Buck et al.(2000) found a clade established by Prionodon densus (Sw. ex Hedw.) Müll. Hal. and Cryphaea glomerata Schimp. ex Sull. Simultaneously, Maeda et al. (2000) cast doubts about the relationship between Cryphaeaceae and Pterobryaceae by finding a polytomy involving Cryphaeaceae and Leucodontaceae. Cox et al. (2010) found a main group of Cryphaeaceae (Cryphaeaceae excluding Dendroalsia abietina, Dendropogonella rufescens (Schimp.) E. Britton, and Pilotrichopsis dentata (Mitt.) Besch.) closely related to an assemblage of non-monophyletic families such as Hypnaceae, Leskeaceae, and Hylocomiaceae among others. The present cladogram (Fig. 2), recovered a clade composed of the paraphyltic Pterobryaceae and Dendroalsia as the sister node of clade A. This pattern, also retrieved in the consensus of all the trees, somehow resembles that of Cox et al. (2010). However, the paraphyletic or polyphyletic condition of many pleurocarpous families makes difficult to establish a reliable sister relationship.
A further significant result of the present study was the non-monophyly of Cryphaea. The definition of this genus has varied since Brotherus (1905) separated Acrocryphaea (= Schoenobryum), Dendrocryphaea and Dendropogon (= Dendropogonella E. Britton) from Cryphaea. After several modifications made by numerous authors, Manuel (1973) transferred C. ravenelii to Cryphaea so that the genus included both species with single and double peristome. Rao (2001) proposed the genus Monocryphaea to accommodate this last species. Consequently, Cryphaea would only consist of species with double peristome. However, as a consequence of the restricted species sampling, this taxonomic proposal and the status of Cryphaea itself could not be rigorously tested. In the present study, a paraphyletic Cryphaea was recovered. In addition, Cryphaea diagnosis might be drastically changed as it embedded Schoenobryum and D. lamyana. This would leave the genus Cryphaea comprised by haplolepidous and diplolepidous species as well as by epiphytic and aquatic species. Hence, the present results as well as those obtained by other authors (Cox et al., 2010) suggest a re-evaluation of Cryphaea as defined by Rao (2001).
As in the case of Cryphaea, the low taxon sampling in previous phylogenetic studies cast doubts about the nature of Dendrocryphaea and Cryphidium. In respect to the latter, there has been a long controversy about its nature since the original definition of the genus (Flores & Suárez, 2014; Jaeger, 1876; Mitten, 1869; Robinson, 1972). Rao (2001), as well as Buck (1980), Suárez and Schiavone (2010) and Flores and Suárez (2014), proposed to keep Cryphidium leucocoleum as such. In the present study, C. leucocoleum appeared as a taxon related to Dendrocryphaea. The position of Dendrocryphaea lamyana is also controversial. Due to its aquatic habitat, Rao (2001) replaced Cryphaea lamyana (Mont.) Müll. Hal. with Dendrocryphaea lamyana. This combination was supported by the results of his phylogenetic analysis, where D. lamyana was sister to Cyptodontopsis leveillei (Rao, 2001). Nevertheless, no other species of Dendrocryphaea was included. Consequently, it was not possible to test the identity of C. lamyana as a Dendrocryphaea in that analysis. Thus, the present results suggest revisiting Rao’s transference. Moreover, on the ground of the current results, Cryphaea lamyana should be revalidated.
The synapomorphies of Cryphaeaceae outlined in the present analysis were, at least partially, associated with the position of Cryphaeophilum molle at the base of the family. Originally, C. molle was recognised as Cryphaea mollis Dusén (Dusén, 1905). This approach was also taken by Brotherus (1903), who placed it within the section Cryphaeopsis Broth. Later, Fleischer (1914) observed differences in leaf anatomy between both genera, and proposed the segregation of C. mollis from the family under the designation of Cryphaeophilum. Because of the association of Cryphaeophilum molle and Cryphaea heteromalla, Quandt et al.(2004) proposed to maintain Cryphaeophilum molle within the Cryphaeaceae. From the present analysis it is clear that C. molle is basally placed within Cryphaeaceae. Thereby, because of both the significant morphological differentiation (Fleischer, 1914; Kühnemann and Gonçalves-Carralves., 1976) and the basal position within the family, the generic distinction between Cryphaeophilum and Cryphaea seems reasonable.
Morphological trends and Diagnosis
Cox et al. (2010) provided the first evidence of a polyphyletic Cryphaeaceae. This non-monophyletic status, retrieved in the present study as well, suggest not only a taxonomic rearrangement but also a revision of the diagnostic characters. Among several features, Cryphaeaceae has long been described as having oval leaf cells, slightly differentiated alar cells, short seta, immersed capsule, papillose peristome teeth throughout, conic operculum, and mitrate calyptra (Buck, 1998; Sharp et al., 1994). Since D. abietina was separated from the other genera of Cryphaeaceae (Fig. 2), most of the apomorphies commonly associated with the family shall be re-defined, discarded, or restricted to groups within Cryphaeaceae. Oval laminal cells were a synapomorphy of the group constituted by the clades B+C. However, the basal position of Cryphaeophilum molle implied that oval cells were not a synapomorphy at familial level. Even so, the group of clades B+C comprised genera with secondarily rhombic (e.g. Cryphaea rhacomitriodes Müll. Hal.) and linear cells (Cryphaea ragazzii (Brizi) Broth., Cryphaea gracillima Herzog). Similarly, weakly differentiated alar cells diagnosed the clade B+C though Cryphaea runtenbergii Müll. Hal., Cryphaea rhacomitriodes and -externally- Cryphaeophilum molle had strongly distinct alar cells. A short seta and an immersed capsule are striking features of the Cryphaeaceae (Buck, 1998; Sharp et al., 1994). The exclusion of D. abietina, whose seta length is 2-2.25 mm, left the clade A diagnosed by a seta of 0.26-0.30 mm in length. Even more, the group B+C is characterized by a shorter seta (0.15-0.27 mm). Posterior increases were found to distinguish nodes at clade B, but none of them was as long as 2 mm. A mitriform calyptra was shown to be a plesiomorphic character state. However, the conical shape of the operculum appeared to be a synapomorphy of the clade A, depicting secondarily rostrate shape in isolated taxa. The papillose peristome and immersed capsule were plesiomorphic character states that originated within the order.
In short, the placement of several species (D. abietina, C. molle, D. lamyana, C. leveillei, S. concavifolium) at unexpected nodes affected the diagnosis of the family and the genus Cryphaea. Aside from the conic operculum and the costa throughout the inner perichaetial bracts; some of the characters were either circumscribed to a narrower morphological span (seta length), restricted to some inner group (oval leaf cells), or discarded as primitive features (mitrate calyptra).
Sources of conflict with previous hypothesis
Given that Rao’s phylogeny (2001) is a cornerstone for the current knowledge of Cryphaea, it is worth trying to determine the sources of discrepancies between that study and the present. The first cause of discrepancy may be related to the wider taxon and character sampling of the present analysis. In addition, Rao used Schoenobryum in order to root the obtained phylogenetic hypothesis. However, no previous phylogenetic evidence supported that choice. To ensure the reliability of choosing an appropriate root terminal (i.e. a taxon that is, in fact, external to Cryphaea and Cryphaeaceae) we decided to select Cyclodictyon (Pilotrichaceae, Hookeriales) for rooting and include species from a large number of related families and orders. Another difference between both analyses lies in the characters included in the matrix, some of them considered for the first time for evaluating this group. In order to include a wider range of outgroup taxa; some of the characters defined by Rao were modified. Along with the differences in the evidence from where the phylogenetic hypotheses are derived, both analyses also differ in their methodologies. For instance, the present study was conducted using weighted parsimony while that of Rao considered equal weights for all characters with the purpose of avoiding bias. Additionally, Rao (2001) kept the multi-state characters as unordered with the intention of minimising assumptions about character evolution. The “unweighted approach” really implies that all the characters are equally capable of the explaining the phylogenetic pattern. As this is certainly not true (due to homoplasy), we used implied weights. Coding multi-state characters as non-additive is equivalent to appreciate the several states within them as being equally different. Obviously, this does not need to be the case. In order to make explicit the similarity among characters states we coded them as ordered in cases where we did observe resemblance (Lipscomb, 1992).
CONCLUSIONS
The aim of this paper was to re-evaluate the relationship of the Cryphaeaceae taxa on the basis of under an improved taxon and character sampling. In addition, several doubtful or previously excluded species were incorporated as well as taxa never before included in phylogenetic studies. As a result of this, the Cryphaeaceae appeared non-monophyletic, Dendroalsia abietina was disaggregated from the family and Cryphaeophilum molle was placed as the sister species of the remaining Cryphaeaceae genera. Contrary to prior hypothesis (Rao, 2001), Cryphaea is paraphyletic. Even though, we take the position of conserving current taxonomic status of Cryphaea until more evidence confirms the findings presented here.
In lights of present results, taxonomical re-arrangements should be considered. Because of the low support and stability of the results we did not take any taxonomical decisions. As can be seen from previous analyses (Cox et al., 2010; Rao, 2001), low character support in phylogenies of pleurocarpous taxa is not a weakness of the current data solely. This tells us about the necessity of looking for new sources of character for the group, such as ultra-structural, developmental, and molecular. Hopefully these new sources of characters will help us to clarify the phylogenetic relationships within Cryphaeaceae and taxonomical identity of its members.
ACKNOWLEDGMENTS
Authors are indebted with herbaria curators (LIL, NY, CTES, MO, BA, RB, HBr, CHR and PC) for providing valuable materials for elaborating this work. Lone Aaegesen and J. Salvador Arias provided invaluable suggestions to improve an early version of the manuscript. TNT is freely available thanks to the Willi Hennig Society.
This study was carried out with the financial support of the National University of Tucumán (CIUNT students’ scholarship and PIUNT G524), the Consejo Nacional de Investigaciones Científicas y Tecnológicas and the Agencia Nacional de Promoción Científica y Tecnológica (PICT 1838, PICT 0810).
SUPPLEMENTARY APPENDIX
SPECIMENS EXAMINED
Cryphaea apiculata Schimp.: M. Schiavone 2535, 3302 (LIL); G. Suárez 482 (LIL); G. Suárez & M. Schiavone 97 (LIL).
Cryphaea brevipila Mitt.: A. Hüebschmann 1 (NY), M. Schiavone et al. 2711(LIL); G. Suárez 162, 522 (LIL); A. Schinini 24788B (NY).
Cryphaea furcinervis Müll. Hal.: M. Schiavone & B. Biasuso 838, 1597 (LIL); G. Suárez & M. Schiavone 49 (LIL).
Cryphaea jamesonii Taylor.: M. Schiavone & B. Biasuso 2154, 3086 (LIL); M. Schiavone, B. Biasuso & S. P. Churchill 2797 (LIL).
Cryphaea lorentziana Müll. Hal.: M. Schiavone & B. Biasuso 1597 (LIL).
Cryphaea patens Hornsch.: G. Suárez 159, 522 (LIL); M. Schiavone & B. Biasuso 837 (LIL); G. Suárez & M. Schiavone 05 (LIL).
Cryphaea rhacomitriodes Müll. Hal.: G. Suárez & M. Schiavone 04,36, 83, 101 (LIL); G. Suárez 151, 152, 153, 166 (LIL); M. Schiavone & B. Biasuso 579, 580, 593, 1366, 2201 (LIL); M. Schiavone 661 (LIL); B. Biasuso 684, 997 (LIL).
Cryphaeophilum molle(Dusén) M. Fleisch.: Dusén 536 (NY, LIL); Dusén 440, 529 (HBr, LIL).
Cryphidium leucocoleum(Mitt.) A. Jaegger.: Lorentz s/n (H-BR) as Cryphaea aurantiorum.; G. Suárez, M. Dematteis, E. Meza-Torres & A. Vega 1337, 1435 (LIL, NY); Sehnem 229 (NY). Neimeyer s/n (NY 01817302).
Cyclodictyon albicans (Hedw.) Kuntze: M. Schiavone 3303 (LIL).
Cyclodictyon lorentzii (Müll.) Buck &Schiavone: M. Schiavone 1265 (LIL).
Cyclodictyon varians (Sull.) Kuntze: Costa et al. 5073 (RB); M. S. Dias s/n (RB 453018).
Dendrocryphaea cuspidata (Sull.) Broth.: Künhnemann 5176 (BA, LIL); Porter 1901 (NY, LIL, HBr); Crosby 11702 (NY, LIL); Dusén 23 (NY, LIL).
Dendrocryphaea gorveana (Mont.) Paris & Schimp.: Montagne s/n (NY, LIL); Lechler s/n (PC, LIL).
Dendrocryphaea tasmanica (Mitt.) Broth.: A. Fife 6725 (CHR, LIL).
Forsstroemia coronata (Mont.) Paris: Pierotti s/n (LIL); G. Suárez & M. Schiavone 15, 35, 86, 88 (LIL); G. Suárez 57, 67, 72 (LIL).
Haplocladium microphyllum (Hedw.) Broth.: William R. Buck 26078 (NY, LIL).
Herpetineuron toccae (Sull. & Lesq.) Cardot: Hosseus 69, 231, 266 (LIL).
Lepidopilum polytrichoides (Hedw.) Brid.: S. P. Churchill & I. Sastre De-Jesús 13744 (NY).
Lepyrodon tomentosus (Hook.) Mitt.: Perez-Moreau s/n (BA, LIL); Aarnokalela b 224 d (BA, LIL); Theirot 1770 (Isotypes: BA, LIL)
Meteoridium remotifolium (Müll.) Manuel: S. P. Churchill & M. Schiavone 20083 (MO).
Meteorium deppei (Müll.) Mitt. : William R. Buck 26042 (LIL); William R. Buck 26087 (NY).
Neckera villa-ricae Besch.: G. Suárez, Dematteis, M, Meza, E & Vega, A 1404, 1419 (LIL, NY); G. Suárez 1123 (LIL); G. Suárez, M. Dematteis, E. Meza, & A. Vega 1228 (CTES, LIL, NY); G. Suárez, M. Dematteis, E. Meza, & A. Vega 1234, 1341 (LIL).
Neckeropsis undulata (Hedw.) Reichardt.: M. Schiavone 3273 (LIL); Michelle J. Price & B. Biasuso et al. 1634 (MO); S. P. Churchill & M. Schiavone 20040 (MO); G. Suárez 150a (LIL).
Orthostichopsis tenuis(A. Jaeger) Broth.: S. P. Churchill, M. Serrano et al. 23660, 23240 (LIL, MO).
Phyllogonium viscosum(P. Beauv.) Mitt.: S. P. Churchill, Marcos Decker & Fabiana Mogro 21070 (LIL); S. P. Churchill, Magombo, Price 19865 (LIL); S. P. Churchill & Toapanta 21035 (LIL).
Prionodon densus (Sw. ex Hedw.) Müll. Hal.: S. P. Churchill & Arroyo 21159 (LIL); S. P. Churchill et al. 23762 (LIL); Schäfer-Verwimp & Verwimp 10018 (LIL).
Pterobryon densumHornsch.: Vargas et al. 1485 (LIL); S. P. Churchill & Arroyo 21226 (LIL).
Rauiella praelonga (Schimp. Ex Besch.) Wijk and Margad.: S. P. Churchill, Serrano et al. 23331 (MO).
Schoenobryum concavifolium(Griff.) Gangulee.: G. Suárez 193, 210, 221, 251 (LIL); M. Schiavone 2536, 53114 (LIL).
Thuidium delicatulum (Hedw.) Schimp.: S. P. Churchill et al. 22068 (MO).
CHARACTERS DESCRIPTION
Description of the new characters included, remaining characters are described in Rao (2001). Continuous and additive characters are marked with “*” and “**”, respectively. Note that measures were made in scale units such as not to exceed the maximum value allowed by TNT (maximum value: 60).
Gametophyte characters:
6.- Stem leaf length (cm)*. The full length of leaves was measured from the apex up to the most basal part of the lamina. In decurrent leaves, wings were also included in total length measure.
7.- Stem leaf width (cm)*. Measured in the widest part of the leaves.
8.- Pseudoparaphyllia extension (mm)*. The length of the pseudoparaphyllia was measured under light microscope along the longest axis, regardless of the pseudoparaphyllia shape.
9.- Alar cell size (micrometer)*. Alar cells size was measured along the longest axis of the cell.
48.- Pseudoparaphyllia: (0) absent; (1) filamentous; (2) foliose.
49.- Paraphyllia: (0) absent; (1) present.
50.- Basal cell of axillary hair: (0) one; (1) two.
51.- Distal cells of axillary hair: (0) one; (1) two; (2) more than two**. Specimens where two states (1 or 2) were observed were exhaustively inspected. Polymorphic scores were considered only when the frequencies of both states were near 50% along more than 10 samples. Otherwise, a single state was scored.
52.- Differentiation of central strand: (0) undifferentiated; (1) differentiated. Central strand is characterized by the presence of elongated (sometimes colourful) thin-walled cells. A typical central strand is observed in Thuidium delicatulum (Hedw.) Schimp.
53.- Leaf dimorphism: (0) no; (1) yes. Dimorphism is evaluated at the same insertion point. A classical dimorphism is found in Cyclodictyon sp. Here, the leaves can be differentiated in shape, ornamentation, placement and/or size.
54.- Differentiation of secondary stem: (0) undifferentiated; (1) differentiated. A secondary stem is differentiated when the main axis (primary stem) turns up near 90° and becomes (generally) devoid of rhizoids. A representative secondary stem is present in most of Cryphaeaceae members.
55.- Hyalodermis: (0) undifferentiated; (1) differentiated.
57.- Condition of stem leaves when dry: (0) contorted;(1) strongly appressed; (2) erect and spreading. The general aspect of stem leaves when dry is a traditional character widely used in bryological taxonomy and is related to strategies to overcome water-stress conditions. A contorted state applies when leaves aspect is tortuous. Strongly appressed leaves are those whose margins are tightly overlapped, as is the case of most Cryphaeaceae. On the other hand, spreading leaves are those whose main axis form a 45° angle with the stem. Pterobryon densum, Lepyrodon tomentosus and Prionodon densus have erect-spreading leaves when dried.
58.- Margins of stem leaves: (0) undifferentiated; (1) differentiated. A differentiated margin is found in some Pilotrichaceae. Leaf margins can be distinguished by being constituted by rows of elongated cells. Those cells are markedly narrower than that of the lamina.
60.- Apex margins of the internal perichaetial bracts (IPB): (0) entire; (1) serrulate. The apex margins condition (entire or serrulate) was examined up to the upper half of the first third of the leaf extension.
66.- Alar cell differentiation: (0) indistinct; (1) slightly differentiated; (2) sharply differentiated. Alar cells tend to be quadrate and sometimes with slightly thick walls. However, alar cells are often inconspicuously differentiated from laminal cells. Cases where alar region is relatively small and cells are hardly defined were considered as state 0. By contrast, a situation where alar region and cells are considerably extended was scored as state 1. In extreme cases, alar cells may not only be distinguished by their shape but also by the color and the thick walls.This last condition (state 2) is distinctive of some species of Meteoriaceae.
67.- Stem (secondary stem)-Branch leaves differentiation: (0) no; (1) yes. When differences in aspect (colour, shape and/or size) were observed between leaves of the branches and the secondary-stem, the character was scored as 1. When no secondary stem was developed, “stem” was simply intended as the main axes.
68.- Creeping secondary stem (stipe) differentiation: (0) indistinct; (1) differentiated. A stipe is defined as an erect secondary stem lacking leaves at its most basal part. This differentiates it from a classical secondary stem of remaining pleurocarpic mosses (e.g Cryphaeaceae).
69.- Leaves of primary and secondary stem: (0) equal; (1) differentiated. As in other characters, the leaves may differ in size, shape or colour.
70.- Stem leaf orientation: (0) spirally; (1) complanate.
71.- Capsule/Perichaetial leaves: (0) immersed; (1) exserted. An exerted capsule is considered as such only when all of its extension fully overcomes the level of the perichaetial leaves. Otherwise, the leaves were considered as immersed.
Sporophyte characters:
46.- Endostome ornamentation: (0) smooth; (1) papillose above, smooth below; (2) entirely papillose. Cases where only the endostome membrane was ornamented, endostome teeth ornamentation was considered as smooth.
47.- Endostome cilia: (0) absent; (1) present.
56.- Endostome cross-walls: (0) depressed; (1) non-depressed. Endostome walls of some Pilotrichaceae are somewhat incurved, giving them a “baffle-like” aspect (sensu Buck 1998). This is distinctive of some species of Cyclodictyon.
59.- Capsule position: (0) erect; (1) sideward or pendent. A sideward capsule was scored as such when the drop (slope) angle fell down 45°.
61.- Exothecial cell form: (0) irregular; (1) somewhat quadrate.
62.- Width of the exothecial cell wall: (0) thin walled; (1) thick walled; (2) collenchymatous.
63.- Aspect of the upper exothecial cells: (0) similar to the lower ones; (1) colour differentiated; (2) form differentiated.
64.- Minimum number of upper exothecial cell rows: (0) one; (1) two; (2) three; (3) four; (4) five**. To establish the minimum number of upper exothecial cells, mode was computed for each species. At least, ten specimens were observed per species.
SENSITIVITY ANALYSIS
Under implied weighting, a concave function is used to define the relationship between homoplasy of each character and the corresponding weight. By changing a concavity constant (K), it is possible to modify how strong homoplasious characters are downweighted. Selection of K values was made in relation to the Fit of a “mean character” (Mirande, 2009); that is, a character with a mean amount of homoplasy over a reference tree (Tree length/number of characters), in this case a cladogram obtained with equal weighted (“non-weighted”) characters. By considering the Fit equation [F: K / (S + K); where S is the step number of a character], the K values chosen were those which assigned to a “mean character” 50, 52, 54, 56, 58, 60, 62, 64, 66 and 68% the fit of a perfectly adjusted binary character (Fig. 1).
COMPARISON OF CONSTRAINED AGAINST NON-CONSTRAINED TREES
In order to compare the score of constrained and non-constrained trees, only one specific fit value of those obtained along the K value range must be considered. To do this, first, a topology (or group of) was selected on the base of its stability (see above). Then, as several trees (and so fit scores) might be identified as equally stable, a middle K value (K = 5.529, in this case) was selected to perform the comparison between constrained and non-constrained trees.
BIBLIOGRAFÍA
Bridel, S. E. 1819. Muscologiae recentiorum supplementum pars IV seu Mantissa Generum Specierumque Muscorum Frondosorum Universa. Lipsiae: Apud. J. A. Barthium.
Brotherus, V. F. 1903. Bryales II: Spezieller Teil., in Engler, A. & Prantl, K. (eds.), Die Natürlichen Pflanzenfamilien nebst ihren Gattungen und wichtigeren Arten, insbesondere den Nutzpflanzen, unter Mitwirkung zahlreicher hervorragender Fachgelehrten begründet I, Abt. III, Halfte I (277-1246). Leipzig: W. Englemann publisher.
Brotherus, V. F. 1905. Cryphaeaceae, Leucodontaceae, in Engler, A. & Prantl, K. (eds.), Die Natürlichen Pflanzenfamilien nebst ihren Gattungen und wichtigeren Arten, insbesondere den Nutzpflanzen, unter Mitwirkung zahlreicher hervorragender Fachgelehrten begründet I, Abt. III Halfte II (736-762). Leipzig: W. Englemann publisher.
Brotherus, V. F. 1924. Musci (Laubmoose), in Engler, A. & Prantl, K. (eds.), Die Natürlichen Pflanzenfamilien nebst ihren Gattungen und wichtigeren Arten, insbesondere den Nutzpflanzen, unter Mitwirkung zahlreicher hervorragender Fachgelehrten begründet 2, Band 11 (1-542). Berlin: Duncker & Humblot publisher.
Buck, W. R. 1980. Animadversions on Pterigynandrum with special commentary on Forsstroemia and Leptopterigynandrum. The Bryologist 83(4): 451-465. DOI: 10.2307/3242298
Buck, W. R. 1988. Another view of familial delimitation in the Hookeriales. Journal of Hattori Botanical Laboratory 64: 29-36.
Buck, W. R. 1998. Pleurocarpous mosses of the West Indies. Memoirs of the New York Botanical Garden 82: 1-400.
Buck, W. R.; B. Goffinet & J. Shaw. 2000. Testing morphological concepts of orders of pleurocarpous mosses (Bryophyta) using phylogenetic reconstructions based on trnL-trnF and rps4 sequences. Molecular Phylogenetics and Evolution 16(2): 180-198. DOI: 10.1006/mpev.2000.0805
Capesius, I. & M. Stech. 1997. Molecular relationships within mosses based in 18S rRNA gene sequences. Nova Hedwigia 64: 525-533.
Cox, C. J. & T. J. Hedderson. 1999. Phylogenetic relationships among the ciliate arthrodontous mosses: Evidence from chloroplast and nuclear DNA sequences. Plant Systematics and Evolution 215(1): 119-139. DOI: 10.1007/BF00984651
Cox, C. J.; B. Goffinet; N. J. Wickett, S. B. Boles & A. J. Shaw. 2010. Moss diversity: a molecular phylogenetic analysis of genera. Phytotaxa 9: 175-195. DOI: 10.11646/phytotaxa.9.1.10
De Luna, E.; A. E. Newton, A. Withey, D. González & B. D. Mishler. 1999. Ordinal phylogeny within the Hypnobryalean Pleurocarpous Mosses inferred from cladistic analyses of three chloroplast DNA sequence data sets: trnL-F, rps4 and rbcL. The Bryologist 103(2): 242-256.
Dusén, P. 1905. Musci nonnulli novi e Fuegia et Patagonia reportati. Botaniska Notiser 11: 299-310.
Farris, J. S.; V. A. Albert, M. Källersjö, D. Lipscomb & A. G. Kluge. 1996. Parsimony jackknifing outperforms neighbor joining. Cladistics 12: 99-124. DOI: 10.1111/j.1096-0031.1996.tb00196.x
Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783-791. DOI: 10.2307/2408678
Fleischer, M. 1906. Die Musci der Flora von Buitenzorg : zugleich Laubmoosflora von Java. Flore de Buitenzorg 5(3): 645-1100. DOI: 10.5962/bhl.title.44870
Fleischer, M. 1914. Kritische Revision von Carl Müllerschen Laubmoosgattungen. Hedwigia 55: 280-285.
Flores, J. R. & G. M. Suárez. 2014. Redescription of the genus Cryphidium (Cryphaeaceae, Bryophyta), with notes on its taxonomy. Boletín de la Sociedad Argentina de Botánica 49(2): 195-199.
Goffinet, B.; W. R. Buck & A. J. Shaw. 2009. Morphology, anatomy and classification of Bryophyta, in Goffinet, B. & A.J. Shaw (eds.), Bryophyte biology. 2nd edition, pp.55-138. Cambridge: Cambridge University Press.
Goloboff, P. A. 1993. Estimating character weights during tree search. Cladistics 9(1): 83-91. DOI: 10.1111/j.1096-0031.1993.tb00209.x
Goloboff, P. A. 1999. Analyzing large data sets in reasonable times: solutions for composite optima. Cladistics 15(4): 415-428. DOI: 10.1111/j.1096-0031.1999.tb00278.x
Goloboff, P. A.; J. S. Farris & K. C. Nixon. 2008a. TNT, a free program for phylogenetic analysis. Cladistics 24(5): 774-786. DOI: 10.1111/j.1096-0031.2008.00217.x
Goloboff, P. A.; J. M. Carpenter, J. S., Arias & D. R. M. Esquivel. 2008b. Weighting against homoplasy improves phylogenetic analysis of morphological data sets. Cladistics 24(5): 758-773. DOI: 10.1111/j.1096-0031.2008.00209.x
Goloboff, P. A.; C. I. Mattoni & A. S. Quinteros. 2006. Continuous characters analyzed as such. Cladistics 22(6): 589-601. DOI: 10.1111/j.1096-0031.2006.00122.x
Gradstein, S. R.; S. P. Churchill & A. N. Salazar. 2001. Guide to the bryophytes of tropical America. Memoirs of the New York Botanical Garden 86: 1-577.
Hedenäs, L. 1994. The basal pleurocarpous diplolepidous mosses: A cladistic approach. The Bryologist 97(3): 225-243. DOI: 10.2307/3243454
Hedenäs, L. 1995. Higher taxonomic level relationships among diplolepidous pleurocarpous mosses—A cladistic overview. Journal of Bryology 18(4): 723-781. DOI: 10.1179/jbr.1995.18.4.723
Hedenäs, L. 1996a. A cladistic evaluation of relationships between the Hookeriales, the Sematophyllaceae and some other taxa. Lindbergia 21(2): 49-82.
Hedenäs, L. 1996b. A cladistic overview of the “Hookeriales” Lindbergia, 21(3): 107-143.
Jaeger, A. 1876. Adumbratio flore muscorum totius orbis terrarum. Part 6. Bericht über die Thätigkeit der St. Gallischen Naturwissenschaftlichen Gesellschaft, 1874-1875: 85-188.
Kühnemann O, Gonçalves Carralves MF. (1976) Sobre Cryphaeophilum molle (Dus.) Fleisch. Meteoriaceae-Briofitas. Boletín de la Sociedad Argentina de Botánica 17(3-4): 247–251. Boletín de la Sociedad Argentina de Botánica 17(3-4): 247–251.
La Farge-England, C. 1996. Growth form, branching pattern, and perichaetial position in mosses: Cladocarpy and pleurocarpy redefined. The Bryologist 99(2): 170-186. DOI: 10.2307/3244546
Lipscomb, D. L. 1992. Parsimony, homology and the analysis of multistate characters. Cladistics 8(1): 45-65. DOI: 10.1111/j.1096-0031.1992.tb00050.x
Maeda, S.; K. Ksuge, D. González, E. De Luna & H. Akiyama. 2000. Molecular phylogeny of the suborder Leucodontineae (Musci; Leucodontales) inferred from rbcL sequence data. Journal of Plant Research 113(1): 29-38. DOI: 10.1007/PL00013900
Manuel, M. G. 1973. Studies in Cryphaeaceae I. A revision of the genus Cryphaea in North America north of Mexico. The Bryologist 76(1): 144-162. DOI: 10.2307/3241235
Manuel, M. G. 1974. A revised classification of the Leucodontaceae and a revision of the subfamily Alsioideae. The Bryologist 77(4): 531-550. DOI: 10.2307/3241800
Manuel, M. G. 1981. Studies in Cryphaeaceae V. A revision of the family in Mexico, Central America and the Caribbean. Journal of Hattori Botanical Laboratory 49: 115-140.
Manuel, M. G. 1982. A brief review of the systematics of the Leucodontaceae and the Cryphaeaceae. Beiheft Zur Nova Hedwigia 71: 281-289.
Mirande, J. M. 2009. Weighted parsimony phylogeny of the family Characidae (Teleostei: Characiformes). Cladistics 25(6): 1-40. DOI: 10.1111/j.1096-0031.2009.00262.x
Mitten, W. 1869. Musci austro-americani. Enumeratio muscorum omnium austroamericanorum auctori hucusque cognitorum. The Journal of the Linnean Society. Botany 12: 1-659.
Quandt, D.; S. Huttunen, H. Streimann, W. Frey & J. P. Frahm. 2004. Molecular phylogenetics of the Meteoriaceae s. str. focusing on the genera Meteorium and Papillaria. Molecular Phylogenetics and Evolution 32(2): 435-461. DOI: 10.1016/j.ympev.2003.12.012
Rao, P. & J. Enroth. 1999. Taxonomic studies on Cryphaea (Cryphaeaceae, Bryopsida) 1. The Chinese species and notes on Cyptodontopsis. Bryobrothera 5: 177-188.
Rao, P. 2000. Taxonomic studies on Cryphaea (Cryphaeaceae, Bryopsida) 2. Revision of Asian species. Annales Botanici Fennici 37(1):45-56.
Rao, P. 2001. A synopsis of the genus Cryphaea (Cryphaeaceae, Bryopsida). Bryobrothera 7: 1–35.
Robinson, H. 1972. The status of the genus Cryphidium. Phytologia 23: 149-150. DOI: 10.5962/bhl.part.19871
Sankoff, D. & P. Rosseau. 1975. Locating the vertices of a Steiner tree in an arbitrary metric space. Mathematical Programming 9(1): 240-246. DOI: 10.1007/BF01681346
Schimper, W.P. 1856. Corollarium Bryologiae Europaeae: conspectum diagnosticum familiarum, generum et specierum, adnotationes novas atque emendationes complectens. Stuttgart: E. Schweizerbart.
Sharp, A. J.; H. Crum & P. M. Eckel. 1994. The moss flora of Mexico. Memoirs of the New York Botanical Garden 69: 1-1113
Suárez, G. M. & M. M. Schiavone. 2004. Comentarios sobre la Posición de Cryphaea furcinervis Müll. Hall y Cryphaea lorentziana Müll. Hall. dentro de Cryphaea (Cryphaeaceae, Musci). V Reunión Argentina de Cladística y Biogeografía. Salta, Argentina.
Suárez, G. M. & Schiavone, M. M. 2010. La familia Cryphaeaceae (Bryophyta) en los bosques del noroeste de Argentina. Boletín de la Sociedad Argentina de Botánica 45: 29-45.
Weber, F. 1813. Tabula exhibens calyptratarum operculatarum sive muscorum frondosorum genera. Kilice: C. F. Mohr.
CLADISTIC ANALYSIS OF THE FAMILY CRYPHAEACEAE (BRYOPHYTA) WITH EMPHASIS ON CRYPHAEA: A STUDY BASED ON A COMPREHENSIVE MORPHOLOGICAL DATASET
Jorge R. Flores; Santiago A. Catalano & Guillermo M. Suárez
bajo Licencia Creative Commons Atribución-NoComercial 2.5 Argentina
Basada en una obra en Revista Darwiniana, nueva serie. Argentina
Programación y Administración: Roberto Donadío