Red tide-forming dinoflagellates from Argentinean coastal waters: insights into Gymnodinium catenatum (Dinophyceae) and first record of G. impudicum

Delfina Aguiar Juárez; Jorge I. Mardones; Luis Norambuena; Javier Paredes-Mella; Eugenia A. Sar; Ana Flores-Leñero; Inés Sunesen

doi:10.14522/darwiniana.2025.131.1273

Authors

Delfina Aguiar Juárez División Ficología Dr. Sebastián Guarrera, Facultad de Ciencias Naturales y Museo (FCNyM), Universidad Nacional de La Plata, Paseo del Bosque s/n, 1900, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290, C1425FQB, Ciudad Autónoma de Buenos Aires, Argentina. https://orcid.org/0000-0002-8733-5107
Jorge I. Mardones Centro de Estudios de Algas Nocivas (CREAN), Instituto de Fomento Pesquero (IFOP), Padre Harter 574, 5480000, Puerto Montt, Chile; Centro de Investigaciones en Recursos Naturales y Sustentabilidad (CIRENYS), Universidad Bernardo O’Higgins, Viel 1497, 8370993, Santiago, Chile. https://orcid.org/0000-0003-3160-0415
Luis Norambuena Centro de Estudios de Algas Nocivas (CREAN), Instituto de Fomento Pesquero (IFOP), Padre Harter 574, 5480000, Puerto Montt, Chile. https://orcid.org/0000-0001-8196-1767
Javier Paredes-Mella Centro de Estudios de Algas Nocivas (CREAN), Instituto de Fomento Pesquero (IFOP), Padre Harter 574, 5480000, Puerto Montt, Chile. https://orcid.org/0000-0002-7035-8150
Eugenia A. Sar División Ficología Dr. Sebastián Guarrera, Facultad de Ciencias Naturales y Museo (FCNyM), Universidad Nacional de La Plata, Paseo del Bosque s/n, 1900, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290, C1425FQB, Ciudad Autónoma de Buenos Aires, Argentina. https://orcid.org/0000-0003-2912-4528
Ana Flores-Leñero Scottish Association for Marine Science, Scottish Marine Institute, Oban, Argyll, PA371QA, Scotland, UK. https://orcid.org/0000-0002-1453-5191
Inés Sunesen División Ficología Dr. Sebastián Guarrera, Facultad de Ciencias Naturales y Museo (FCNyM), Universidad Nacional de La Plata, Paseo del Bosque s/n, 1900, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290, C1425FQB, Ciudad Autónoma de Buenos Aires, Argentina. https://orcid.org/0000-0003-3219-456X

DOI:

https://doi.org/10.14522/darwiniana.2025.131.1273

Keywords:

Atlantic Ocean, Harmful Algal Blooms (HAB), Paralytic Shellfish Toxins (PST), Phylogeny, Toxicology

Abstract

As a part of a monitoring program of harmful microalgae in the coastal waters of Buenos Aires Province, two strains of chain-forming Gymnodinium species (Dinophyceae), LPCc043 and LPCc044, were isolated from samples collected in Samborombón Bay. This study aims to provide a morphological, phylogenetic and toxicological characterization of the strains. Among marine chain-forming Gymnodinium species, only G. catenatum and G. impudicum are known to form harmful algal blooms (HAB). Morphological analysis with light and electron scanning microscopy (LM, SEM) revealed that strain LPCc043 had larger cells, a higher number of cells per chain, and an acrobase independent of the sulcus entering the epicone, whereas strain LPCc044 had smaller cells, a lower number of cells per chain, and an acrobase as an extension of the sulcus. Phylogenetic analysis based on partial LSU rDNA sequencing placed the sequence corresponding to strain LPCc043 in the G. catenatum clade and that corresponding to strain LPCc044 in the G. impudicum clade with a 99–1.0 and 100–1.0 bootstrap and Bayesian posterior probability, respectively. Toxicological analysis by hydrophilic interaction liquid chromatography with tandem mass spectrometry (HILIC-UHPLC-MS/MS) detected paralytic shellfish toxins (PSTs) in G. catenatum, predominantly N-sulfocarbamoyl-11-hydrosulfate toxin 1/2 (C1/C2) and decarbamoyl gonyautoxin 2/3 (dcGTX2/3). In contrast, no PST were found in G. impudicum. This study contributes to the knowledge of harmful algal species diversity along the Argentinean coast and highlights the importance of monitoring these species for public health and environmental management.

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